Elabscience

Lactate Dehydrogenase (LDH) Activity Assay Kit

Product Code:
 
E-BC-K046-M
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Shipping:
 
Ice packs
Storage:
 
2-8°C
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E-BC-K046-M-96T96T£177.00
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E-BC-K046-M-500Assays500Assays£344.00
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  • Further Information
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Further Information

Assay Time:
45 min
Detection Instrument:
Microplate reader
Detection Range:
6-1000 U/L
Detection Type:
Colorimetric method
Inter cv:
2.4
Intra cv:
1.8
Result Type:
Enzyme Activity
Sensitivity:
6 U/L
Target Synonym:
LDH
Test Principle:
Using coenzyme I as a hydrogen carrier, LDH catalyze lactic acid to produce pyruvate. Pyruvate reacted with 2, 4-dinitrophenylhydrazine to form pyruvate dinitrophenylhydrazone, which was red-brown in alkaline solution, and the color depth was proportional to pyruvate concentration. The activity of LDH could be calculated by measuring OD value.
Tested Sample Types:
Serum,plasma,hydrothorax,tissue,cells

Documents

References

  • https://www.sciencedirect.com/science/article/pii/S004896972105405X
  • https://www.sciencedirect.com/science/article/pii/S0141813022004615
  • https://www.sciencedirect.com/science/article/pii/S0278691522002599
  • https://www.mdpi.com/1861292
  • https://www.sciencedirect.com/science/article/pii/S2212429222005399
  • https://europepmc.org/article/med/34803688?javascript_support=no
  • https://www.sciencedirect.com/science/article/pii/S1357272521000819
  • https://www.mdpi.com/article/10.3390/vaccines10081335
  • https://www.nature.com/articles/s41598-022-24556-0
  • https://www.worldscientific.com/doi/abs/10.1142/S0192415X21500452
  • https://onlinelibrary.wiley.com/doi/abs/10.1002/cbin.11897
  • https://www.sciencedirect.com/science/article/pii/S0014299921005069