Elabscience

Glucose-6-Phosphate Dehydrogenase (G-6-PD) Activity Assay Kit

Product Code:
 
E-BC-K056-M
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Shipping:
 
Ice packs
Storage:
 
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E-BC-K056-M-96T96T£177.00
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Further Information

Assay Time:
20 min
Detection Instrument:
Microplate reader
Detection Range:
0.01-50 U/L
Detection Type:
Colorimetric method
Inter cv:
5.8
Intra cv:
2.1
Result Type:
Enzyme Activity
Sensitivity:
0.01 U/L
Target Synonym:
G-6-PD;G6PDH
Test Principle:
Under the presence of G6PDH, glucose-6-phosphoric acid is oxidized to 6-PG. In this reaction, NADP+ is reduced to NADPH. Under the action of electron coupling reagent 1-MPMS, NADPH reducts wST-8 to form orange formazan, which has the maximum absorption peak at about 450 nm. Formazan generated in the reaction system is proportional to the activity of G6DPH in the sample.
Tested Sample Types:
Serum, plasma, animal tissue

Documents

References

  • https://www.sciencedirect.com/science/article/pii/S0378113521001048