Elabscience

Homocysteine (Hcy) Colorimetric Assay Kit (Enzyme Circulation Method)

Product Code:
 
E-BC-K143
Product Group:
 
Biochemical Assay Kits
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Shipping:
 
Ice packs
Storage:
 
2-8°C
 

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CodeSizePrice
E-BC-K143-100Assays100Assays£518.00
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  • Further Information
  • Documents
  • References
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Further Information

Assay Time:
25 min
Detection Instrument:
Biochemistry analyzer, Spectrophotometer
Detection Range:
0-50 umol/L
Detection Type:
Colorimetric method
Result Type:
Quantitative
Target Synonym:
Hcy
Test Principle:
Oxidized homocysteine (HCY) is reduced to free homocysteine by triethyl phosphine (TCEP), and the free homocysteine reacts with substrate to generate adenosine. The generated adenosine is immediately dehydrogenated into inosine and ammonia, and the ammonia is further react with NADH under the catalysis of glutamate dehydrogenase to convert NADH to NAD+. The decrease in absorbance at 340 nm caused by the decline of NADH is proportional to the concentration of homocysteine in the sample.
Tested Sample Types:
Serum

Documents

References

  • https://pubs.rsc.org/en/content/articlelanding/2022/fo/d1fo03403k
  • https://www.mdpi.com/1422-0067/22/21/11737
  • https://www.mdpi.com/2218-273X/12/5/735
  • https://link.springer.com/article/10.1007/s11596-021-2379-1
  • https://onlinelibrary.wiley.com/doi/abs/10.1002/jcla.23996
  • https://www.sciencedirect.com/science/article/pii/S0002962921002469
  • https://www.sciencedirect.com/science/article/pii/S0166432818312646
  • https://www.sciencedirect.com/science/article/pii/S1052305720309551
  • https://link.springer.com/article/10.1134/S181971242203014X
  • https://www.ajol.info/index.php/ahs/article/view/195031
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520594/