Elabscience

Aspartate Amino transferase (AST/GOT) Activity Assay Kit (Reitman-Frankel Method)

Product Code:
 
E-BC-K236-S
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Shipping:
 
Ice packs
Storage:
 
2-8°C
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E-BC-K236-S-100assays100 assays£197.00
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  • Further Information
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Further Information

Assay Time:
100 min
Detection Instrument:
Spectrophotometer
Detection Range:
0.38-72.30 IU/L
Detection Type:
Colorimetric method
Inter cv:
4.9
Intra cv:
4.2
Result Type:
Enzyme Activity
Sensitivity:
0.38 IU/L
Target Synonym:
AST;GOT
Test Principle:
AST enables alpha-ketoglutaric acid and aspartic acid to displace amino to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form Pyroracemic acid during the reaction. Pyroracemic acid reacted with 2,4-dinitrophenylhydrazine (DNPH) to form 2,4-dinitrophenylhydrazone showing reddish brown in alkaline solution. Measure the OD values and calculate the enzyme activity.
Tested Sample Types:
Serum, plasma, animal tissue

Documents

References

  • https://aasldpubs.onlinelibrary.wiley.com/doi/abs/10.1002/hep.29573
  • https://pubs.acs.org/doi/abs/10.1021/acs.est.2c00516
  • https://www.sciencedirect.com/science/article/pii/S0304389420328673
  • https://pubs.acs.org/doi/abs/10.1021/acsami.1c12235
  • https://www.sciencedirect.com/science/article/pii/S2211124722011457
  • https://onlinelibrary.wiley.com/doi/abs/10.1111/cpr.13332
  • https://www.sciencedirect.com/science/article/pii/S1742706121007108
  • https://pubs.acs.org/doi/abs/10.1021/acsami.9b19518
  • https://www.mdpi.com/1891970
  • https://www.sciencedirect.com/science/article/pii/S0753332222001214
  • https://www.mdpi.com/1861292
  • https://www.sciencedirect.com/science/article/pii/S2212429222005399