Elabscience

Sucrase Activity Assay Kit

Product Code:
 
E-BC-K751-M
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Shipping:
 
Ice packs
Storage:
 
2-8°C
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E-BC-K751-M-96T96T£291.00
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Further Information

Assay Time:
70 min
Detection Instrument:
Microplate reader
Detection Range:
20-2000 U/mL
Detection Type:
Colorimetric method
Inter cv:
6.5
Intra cv:
5.4
Result Type:
Enzyme Activity
Sensitivity:
20 U/mL
Test Principle:
Sucrase catalyzes its substrate (sucrose) to produce glucose, which produces hydrogen peroxide under the action of glucose oxidase. Hydrogen peroxide reacts with chromogenic agent to produce red substance, which has a strong absorption peak at 505 nm. In a certain concentration range, It's absorbance is proportional to glucose concentration. Therefore, the activity of sucrase can be calculated by measuring the OD value at 505 nm.
Tested Sample Types:
animal tissue

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