Recombinant Human MAP4K2/GC Kinase Protein (His & GST Tag)

MAP4K2;GC Kinase

AAH47865.1

Mitogen-activated protein kinase kinase kinase kinase 2, also known as B lymphocyte serine/threonine-protein kinase, Germinal center kinase, MAPK/ERK kinase kinase kinase 2, MEK kinase kinase 2, Rab8-interacting protein and MAP4K2, is a cytoplasm and peripheral membrane protein which belongs to theprotein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. MAP4K2 contains oneCNH domain and oneprotein kinase domain. Although this kinase is found in many tissues, its expression in lymphoid follicles is restricted to the cells of germinal centre, where it may participate in B-cell differentiation. MAP4K2 can be activated by TNF-alpha, and has been shown to specifically activate MAP kinases. It is also found to interact with TNF receptor-associated factor 2 (TRAF2), which is involved in the activation of MAP3K1 / MEKK1. MAP4K2 enhances MAP3K1 oligomerization, which may relieve amino-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. It may also play a role in the regulation of vesicle targeting or fusion.

119 kDa

< 1.0 EU per ug of the protein as determined by the LAL method.

Baculovirus-Insect Cells

Supplied as sterile solution of 50mM Tris, 100mM NaCl, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

N-His & GST

116 kDa

> 87 % as determined by reducing SDS-PAGE.

Met 1-Tyr 812

BL44;GCK;MAP4K2;RAB8IP