NSJ Bioreagents

TIMP2 Antibody

Product Code:
 
NSJ-V3899SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
TIMP2/2044
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the TIMP2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
IHC testing of FFPE human placenta with TIMP2 antibody (clone TIMP2/2044). Required HIER: requires boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.~
2 / 2
Analysis of HuProt(TM) microarray
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IHC testing of FFPE human placenta with TIMP2 antibody (clone TIMP2/2044). Required HIER: requires boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.~
Analysis of HuProt(TM) microarray

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NSJ-V3899SAF-100UG100 ug£534.00
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Further Information

Application Details:
ELISA: 2-4ug/ml (order BSA/azide-free format),Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TIMP2 antibody to be titered up or down for optimal performance.
Description:
It recognizes a protein of 21kDa, identified as tissue inhibitor of metalloproteinases 2 (TIMP2). It is closely related to TIMP1 and shows the highest binding affinity to both the latent (pro) and active forms of 72kDa Type IV collagenase (also known as MMP2 or gelatinase A). It also has affinity for the active form of 92kDa Type IV collagenase (also known as MMP9 or gelatinase B). TIMP2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 48-220 from the human protein was used as the immunogen for this TIMP2 antibody.
Limitation:
This TIMP2 antibody is available for research use only.
Localization:
Cytoplasmic, cell surface, secreted
Purity:
Protein G affinity chromatography
Uniprot #:
P16035

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