NSJ Bioreagents

TGF alpha Antibody Cocktail

Product Code:
 
NSJ-V2887SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
TG86 + P/T1
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the TGF-alpha antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC: Formalin-fixed, paraffin-embedded human testicular carcinoma stained with TGF-alpha antibody (TG86 + P/T1).
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IHC: Formalin-fixed, paraffin-embedded human testicular carcinoma stained with TGF-alpha antibody (TG86 + P/T1).

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NSJ-V2887SAF-100UG100 ug£534.00
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Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT
Application Note:
Optimal dilution of the TGF-alpha antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Description:
This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this mAb is completely blocked by the peptide used for raising this antibody. TGFa is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Amino acids 34-43 (PPVAAAVVSH) from the human protein were used as the immunogen for the TGF-alpha antibody.
Limitation:
This TGF-alpha antibody cocktail is available for research use only.
Localization:
Cytoplasmic and extracellular (secreted)
Purity:
PEG precipitation
Uniprot #:
P01135

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