E-Cadherin Antibody / CDH1

Immunofluorescence: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Optimal dilution of the E-Cadherin antibody should be determined by the researcher.

Recognizes a protein of 80-120kDa, identified as E-cadherin/CDH1. Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as b-catenin, to regulate cadherin function. E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin +) and lobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

A recombinant human protein was used as the immunogen for the E-Cadherin antibody.

This E-Cadherin antibody is available for research use only.

Cytoplasmic, membranous

Protein G affinity chromatography

P12830