NSJ Bioreagents

Biotin / Vitamin B7 / Vitamin H Antibody

Product Code:
 
NSJ-V9113SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
SPM375
Regulatory Status:
 
RUO
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the anti-Biotin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC: Formalin-fixed, paraffin-embedded human tonsil stained with biotinylated Lambda Light Chain Ab probe followed by anti-Biotin antibody (SPM375).
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IHC: Formalin-fixed, paraffin-embedded human tonsil stained with biotinylated Lambda Light Chain Ab probe followed by anti-Biotin antibody (SPM375).

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NSJ-V9113SAF-100UG100 ug£534.00
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Biotinylated sheep immunoglobulin was used as the immunogen for this anti-Biotin antibody.
Limitation:
This anti-Biotin antibody is available for research use only.
Localization:
target location
Purity:
Protein G affinity chromatography

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