NSJ Bioreagents

PCNA Antibody

Product Code:
 
NSJ-V9064IHC
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2a kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
SPM350
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the anti-PCNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC analysis of formalin-fixed, paraffin-embedded human tonsil stained with anti-PCNA antibody (clone SPM350).
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IHC analysis of formalin-fixed, paraffin-embedded human tonsil stained with anti-PCNA antibody (clone SPM350).

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NSJ-V9064IHC-7ML7 ml£534.00
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Further Information

Application Details:
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases.
Format:
Purified
Formulation:
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Immunogen:
Rat PCNA protein was used as the immunogen for this anti-PCNA antibody.
Limitation:
This anti-PCNA antibody is available for research use only.
Localization:
Predominantly nuclear, some cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P12004

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