NSJ Bioreagents

Bax Antibody

Product Code:
 
NSJ-V9071SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
SPM336
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the anti-Bax antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 4
Immunofluorescent staining of fixed human Jurkat cells with anti-Bax antibody (clone SPM336, green) and NucSpot nuclear stain (red).
2 / 4
Western blot testing of HeLa cell lysate with anti-Bax antibody (clone SPM336). Observed molecular weight ~21 kDa.
3 / 4
IHC analysis of formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with anti-Bax antibody (clone SPM336).
4 / 4
Flow cytometry testing of fixed human Jurkat cells with anti-Bax antibody (clone SPM336); Red=isotype control, Blue= anti-Bax antibody.
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Immunofluorescent staining of fixed human Jurkat cells with anti-Bax antibody (clone SPM336, green) and NucSpot nuclear stain (red).
Western blot testing of HeLa cell lysate with anti-Bax antibody (clone SPM336). Observed molecular weight ~21 kDa.
IHC analysis of formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with anti-Bax antibody (clone SPM336).
Flow cytometry testing of fixed human Jurkat cells with anti-Bax antibody (clone SPM336); Red=isotype control, Blue= anti-Bax antibody.

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NSJ-V9071SAF-100UG100 ug£534.00
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Further Information

Application Details:
Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
Recognizes a protein of 21kDa, identified as the Bax protein. This mAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Amino acids 3-16 (GSGEQPRGGGPTSS) from the human protein were used as the immunogen for this anti-Bax antibody.
Limitation:
This anti-Bax antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
Q07812

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