NSJ Bioreagents

Glycophorin A Antibody

Product Code:
 
NSJ-V3725SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
SGPA35-2R
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the recombinant Glycophorin A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
IHC staining of FFPE human angiosarcoma with recombinant Glycophorin A antibody (clone SGPA35-2R). Required HIER: steam section in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool prior to staining.~
2 / 2
Western blot testing of huma
❮❯

IHC staining of FFPE human angiosarcoma with recombinant Glycophorin A antibody (clone SGPA35-2R). Required HIER: steam section in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool prior to staining.~
Western blot testing of huma

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NSJ-V3725SAF-100UG100 ug£534.00
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Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Glycophorin A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Glycophorin A (MNS blood group), also known as GYPA, is a protein which in humans is encoded by the GYPA gene. GYPA has also been designated CD235a. Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. [Wiki]
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant human GYPA protein was used as the immunogen for the recombinant Glycophorin A antibody.
Limitation:
This recombinant Glycophorin A antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Protein A affinity chromatography
Uniprot #:
P02724

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