NSJ Bioreagents

Human IgA1 Antibody

Product Code:
 
NSJ-R20183BTN
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
RM124
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
Storage:
 
Store the recombinant Human IgA1 antibody at -20oC.
1 / 5
Sandwich ELISA of human IgA1 using the recombinant Human IgA1 antibody as the capture (100ng/well), and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody clone RM129</a> as the detect, followed by an alkaline phosphatase conjugated streptavidin.
2 / 5
Sandwich ELISA of human plasma using the recombinant human plasma antibody as the capture (100ng/well), and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody clone RM129</a> as the detect, followed by an alkaline phosphatase conjugated streptavidin.
3 / 5
IHC testing of FFPE human tonsil tissue with recombinant Human IgA1 antibody. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
4 / 5
ELISA of human immunoglobulins shows the recombinant Human IgA1 antibody reacts only to Human IgA. Very slightly cross reacts with IgA2. No cross reactivity with human IgG, IgM, IgD, or IgE.
5 / 5
ELISA Titration: the plate was coated with different amounts of human IgA1. A serial dilution of recombinant Human IgA1 antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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Sandwich ELISA of human IgA1 using the recombinant Human IgA1 antibody as the capture (100ng/well), and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody clone RM129</a> as the detect, followed by an alkaline phosphatase conjugated streptavidin.
Sandwich ELISA of human plasma using the recombinant human plasma antibody as the capture (100ng/well), and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody clone RM129</a> as the detect, followed by an alkaline phosphatase conjugated streptavidin.
IHC testing of FFPE human tonsil tissue with recombinant Human IgA1 antibody. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
ELISA of human immunoglobulins shows the recombinant Human IgA1 antibody reacts only to Human IgA. Very slightly cross reacts with IgA2. No cross reactivity with human IgG, IgM, IgD, or IgE.
ELISA Titration: the plate was coated with different amounts of human IgA1. A serial dilution of recombinant Human IgA1 antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

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CodeSizePrice
NSJ-R20183BTN-50UG50ug£524.00
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Further Information

Application Details:
ELISA: 50ng/well - 200ng/well (Capture); 0.05-0.2ug/ml (Detection),Immunocytochemistry: 0.5-2ug/ml,Immunohistochemistry: 0.1-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Human IgA1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant Human IgA1 antibody reacts to human IgA1, and very slightly cross reacts with IgA2. No cross reactivity with human IgG, IgM, IgD, or IgE.
Format:
Biotin Conjugate
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Human IgA was used as the immunogen for this recombinant Human IgA1 antibody.
Limitation:
This recombinant Human IgA1 antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P01876

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