NSJ Bioreagents

Estrogen Receptor alpha Antibody

Product Code:
 
NSJ-V3687SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
NR3Ga-4R
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the recombinant Estrogen Receptor alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
IHC staining of FFPE human breast cancer with Estrogen Receptor alpha antibody (clone NR3Ga-4R). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min. ~
2 / 2
Western blot testi
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IHC staining of FFPE human breast cancer with Estrogen Receptor alpha antibody (clone NR3Ga-4R). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min. ~
Western blot testi

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NSJ-V3687SAF-100UG100 ug£534.00
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Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Estrogen Receptor alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Estrogen receptor (ER) is an important regulator of growth and differentiation in the mammary gland. ER presence in breast tumors indicates an increased likelihood of response to anti-estrogen therapy.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Human Estrogen Receptor alpha protein was used as the immunogen for this recombinant Estrogen Receptor alpha antibody.
Limitation:
This recombinant Estrogen Receptor alpha antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein A affinity chromatography
Uniprot #:
P03372

Documents