NSJ Bioreagents

Nucleolin Antibody

Product Code:
 
NSJ-V7196IHC
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
NPC23-2
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the Nucleolin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 1
IHC testing of FFPE human tonsil with Nucleolin antibody (clone NPC23-2). FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
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IHC testing of FFPE human tonsil with Nucleolin antibody (clone NPC23-2). FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.

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NSJ-V7196IHC-7ML7 ml£534.00
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This product comes from: United States.
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Further Information

Application Details:
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Application Note:
Titering of the Nucleolin antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Nucleolin (NCL) is the major nucleolar phosphoprotein of growing eukaryotic cells. It is located mainly in dense fibrillar regions of the nucleolus. NCL is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.
Format:
Purified
Formulation:
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Immunogen:
Recombinant human protein was used as the immunogen for the Nucleolin antibody.
Limitation:
This Nucleolin antibody is available for research use only.
Localization:
Nucleoli
Purity:
Protein G affinity chromatography
Uniprot #:
P19338

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