NSJ Bioreagents

Moesin Antibody

Product Code:
 
NSJ-V2719SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
MSN/493
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the Moesin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 6
IHC: Formalin-fixed, paraffin-embedded human placenta stained with Moesin antibody (MSN/493). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 6
IHC: Formalin-fixed, paraffin-embedded human testicular carcinoma stained with Moesin antibody (MSN/493). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 6
Western blot testing of human PC3 cell lysate with Moesin antibody. Predicted molecular weight ~68 kDa but routinely observed at 68-78 kDa.
4 / 6
Immunofluorescent staining of PFA-fixed human HeLa cells with Moesin antibody (clone MSN/493, green) and Reddot nuclear stain (red).
5 / 6
Flow cytometry testing of PFA-fixed human K562 cells with Moesin antibody (clone MSN/493); Red=isotype control, Blue= Moesin antibody.
6 / 6
SDS-PAGE Analysis of Purified, BSA-Free Moesin Antibody (clone MSN/493). Confirmation of Integrity and Purity of the Antibody.

IHC: Formalin-fixed, paraffin-embedded human placenta stained with Moesin antibody (MSN/493). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC: Formalin-fixed, paraffin-embedded human testicular carcinoma stained with Moesin antibody (MSN/493). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human PC3 cell lysate with Moesin antibody. Predicted molecular weight ~68 kDa but routinely observed at 68-78 kDa.
Immunofluorescent staining of PFA-fixed human HeLa cells with Moesin antibody (clone MSN/493, green) and Reddot nuclear stain (red).
Flow cytometry testing of PFA-fixed human K562 cells with Moesin antibody (clone MSN/493); Red=isotype control, Blue= Moesin antibody.
SDS-PAGE Analysis of Purified, BSA-Free Moesin Antibody (clone MSN/493). Confirmation of Integrity and Purity of the Antibody.

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NSJ-V2719SAF-100UG100 ug£534.00
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This product comes from: United States.
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Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 2-4ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the Moesin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
Recognizes 78kDa moesin protein. Moesin, a member of the talin-4.1 superfamily, is a linking protein of the sub-membranous actin cytoskeleton. It is expressed in variable amounts in cells of different phenotypes such as macrophages, lymphocytes, fibroblastic, endothelial, epithelial, and neuronal cell lines but not in blood cells. The ERM proteins, ezrin, radixin, and moesin are involved in a variety of cellular functions, such as cell adhesion, migration, and the organization of cell surface structures, and are highly homologous, both in protein sequence and in functional activity, with merlin/schwannomin, a neurofibromatosis-2-associated tumor-suppressor protein. Cell lines of epithelial and mesothelial origin contain both moesin and radixin whereas cells of endothelial and lymphoid origin express moesin.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human protein was used as the immunogen for the Moesin antibody.
Limitation:
This Moesin antibody is available for research use only.
Localization:
Cytoplasmic & cell surface
Purity:
Protein G affinity chromatography
Uniprot #:
P26038

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