NSJ Bioreagents

Cytokeratin 1 Antibody

Product Code:
 
NSJ-V7907SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2a kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
LHK1
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the Cytokeratin 1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 3
IHC staining of FFPE human cervix with Cytokeratin 1 antibody (clone LHK1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 3
IHC staining of FFPE human skin with Cytokeratin 1 antibody (clone LHK1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free Cytokeratin 1 antibody (clone LHK1) as confirmation of integrity and purity.
❮❯

IHC staining of FFPE human cervix with Cytokeratin 1 antibody (clone LHK1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human skin with Cytokeratin 1 antibody (clone LHK1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Cytokeratin 1 antibody (clone LHK1) as confirmation of integrity and purity.

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NSJ-V7907SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 0.1-0.2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Cytokeratin 1 is a hetero-tetramer of two type I as well as two type II keratins, keratin-10 (KRT10). Keratin 1 is differentiation specific keratin that is one of the predominant keratins in suprabasal keratinocytes in stratified epithelia. Mutations in keratin 1 cause epidermolytic hyperkeratosis.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Amino acids VRFVSTSYSGVTR from the C-terminal portion of the protein was used as the immunogen for this Cytokeratin 1 antibody.
Limitation:
This Cytokeratin 1 antibody is available for research use only.
Purity:
Protein G affinity chromatography

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