NSJ Bioreagents

Collagen VII antibody / COL7A1

Product Code:
 
NSJ-V7688SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
LH7.2
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the Collagen VII antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 5
IHC staining of FFPE human skin with Collagen VII antibody (clone LH7.2). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human skin with Collagen VII antibody (clone LH7.2). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
3 / 5
Immunofluorescent staining of PFA-fixed human HeLa cells with Collagen VII antibody (clone LH7.2, green) and Reddot nuclear stain (red).
4 / 5
Flow cytometry testing of PFA-fixed human HeLa cells with Collagen VII antibody (clone LH7.2); Red=isotype control, Blue= LH7.2 antibody.
5 / 5
SDS-PAGE analysis of purified, BSA-free Collagen VII antibody (clone LH7.2) as confirmation of integrity and purity.
❮❯

IHC staining of FFPE human skin with Collagen VII antibody (clone LH7.2). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE human skin with Collagen VII antibody (clone LH7.2). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Immunofluorescent staining of PFA-fixed human HeLa cells with Collagen VII antibody (clone LH7.2, green) and Reddot nuclear stain (red).
Flow cytometry testing of PFA-fixed human HeLa cells with Collagen VII antibody (clone LH7.2); Red=isotype control, Blue= LH7.2 antibody.
SDS-PAGE analysis of purified, BSA-free Collagen VII antibody (clone LH7.2) as confirmation of integrity and purity.

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NSJ-V7688SAF-100UG100 ug£534.00
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Further Information

Application Details:
ELISA (order BSA-free format for coating),Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
Recognizes an epitope located on collagenase digested Type VII collagen (150kDa), i.e. the non-helical carboxyl terminal region of the dimer. Binding is localized at the inferior border of the lamina densa as demonstrated by immunoelectron microscopy. Type VII is also found in the retina, where its function is unknown. It interacts with Laminin 5 and Fibronectin. Collagen VII is a stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A recombinant full-length human Collagen VII protein was used as the immunogen for this Collagen VII antibody.
Limitation:
This Collagen VII antibody is available for research use only.
Purity:
Protein G affinity chromatography
Uniprot #:
Q02388

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