NSJ Bioreagents

CD25 Antibody / IL2RA

Product Code:
 
NSJ-V3849SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
IL2RA/2395
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the CD25 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC testing of FFPE human tonsil tissue with CD25 antibody (clone IL2RA/2395). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.~
2 / 2
Analysis of HuProt(TM) microarray containing more
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IHC testing of FFPE human tonsil tissue with CD25 antibody (clone IL2RA/2395). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.~
Analysis of HuProt(TM) microarray containing more

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NSJ-V3849SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT
Application Note:
Optimal dilution of the CD25 antibody should be determined by the researcher.
Description:
Recognizes a protein of 55 kDa, identified as CD25. It is expressed on activated T- and B-cells and activated monocytes/macrophages. With respect to lymphomas, CD25 is present on malignant cells of Hodgkins disease, HTLV-1+ adult T-cell leukemia, cutaneous T-cell lymphoma, and hair cell leukemia. Increased levels of soluble CD25 are observed in the leukemias/lymphomas and inflammatory/ autoimmune diseases. CD25 molecule alone appears to function as a low affinity receptor and associates with CD122 (IL-2R b-chain, p75) and CD132 (common g-chain) to form the high affinity IL-2 receptor complex. CD25 antibodies detect three epitope regions, A, B and C. This mAb recognizes the epitope B, which is located at residue 3-104 of CD25 and does not block IL-2 binding to CD25.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 42-183 was used as the immunogen for the CD25 antibody.
Limitation:
This CD25 antibody is available for research use only.
Localization:
Cell surface, Cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P01589

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