NSJ Bioreagents

POU2AF1 Antibody / BOB-1

Product Code:
 
NSJ-V7324SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
BOB1/2421
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the BOB1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Western blot testing of human Raji cell lysate with BOB1 antibody (clone BOB1/2421). Predicted molecular weight: ~28 kDa (unmodified), 35-40 kDa (ubiquitinated).~
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IHC testing of FFPE human spleen with BOB1 antibody (clone BOB1/2421). HIE
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Western blot testing of human Raji cell lysate with BOB1 antibody (clone BOB1/2421). Predicted molecular weight: ~28 kDa (unmodified), 35-40 kDa (ubiquitinated).~
IHC testing of FFPE human spleen with BOB1 antibody (clone BOB1/2421). HIE

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NSJ-V7324SAF-100UG100 ug£534.00
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Further Information

Application Details:
Flow cytometry: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the BOB1 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
BOB1, also called POU2AF1, expression in a variety of established B-cell lines, representing different stages of B-cell development, has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immuno-positive for BOB1. Conversely, only some cases of classical Hodgkin lymphoma show BOB1 immuno-reactivity within the Hodgkin and Reed-Sternberg cells. Expression of BOB1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia. B-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 148-255 from the human protein was used as the immunogen for the BOB1 antibody.
Limitation:
This BOB1 antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity
Uniprot #:
Q16633

Documents