NSJ Bioreagents

CD22 Antibody / BL-CAM

Product Code:
 
NSJ-V8594SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
BLCAM/2637R
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the recombinant CD22 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC staining of FFPE human tonsil with recombinant CD22 antibody (clone BLCAM/2637R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~
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IHC staining of FFPE human spleen with recombi
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IHC staining of FFPE human tonsil with recombinant CD22 antibody (clone BLCAM/2637R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~
IHC staining of FFPE human spleen with recombi

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NSJ-V8594SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Application Note:
Optimal dilution of the recombinant CD22 antibody should be determined by the researcher.
Description:
Recognizes a protein of 130-140kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 52-178 from the human protein was used as the immunogen for the recombinant CD22 antibody.
Limitation:
This recombinant CD22 antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein A affinity chromatography
Uniprot #:
P20273

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