EGF Receptor / EGFR Antibody Cocktail
Product Code:
NSJ-V2488IHC
Antibody Isotype:
Mouse IgG2a kappa
Antibody Clonality:
Monoclonal
Antibody Clone:
31G7 + GFR1195
Application:
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the EGFR antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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This product comes from:
United States.
Typical lead time:
10-14 working days.
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Further Information
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Optimal dilution of the EGFR antibody cocktail should be determined by the researcher.
1. Digest formalin-fixed tissues with Protease at 1mg/ml PBS, pH 7.4 for 10 min at 37oC
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
This mAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.
Purified
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Human EGF Receptor purified from A431 cells (31G7) and recombinant human protein (GFR1195) were used as the immunogen for the EGFR antibody cocktail.
This EGFR antibody cocktail is available for research use only.
Cell surface
Protein G affinity chromatography
P00533
