NSJ Bioreagents

CD45RO Antibody (T-cell marker)

Product Code:
 
NSJ-V2416SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2a kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
190-2F2.5
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
 
The CD45RO antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.
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Immunofluorescent staining of PFA-fixed human Jurkat cells with CD45RO antibody (clone 190-2F2.5, green) and Reddot nuclear stain (red).~
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Flow cytometry staining of PFA-fixed human Jukat cells with CD45RO antibody (clone 190-2F2.5); Red=
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Immunofluorescent staining of PFA-fixed human Jurkat cells with CD45RO antibody (clone 190-2F2.5, green) and Reddot nuclear stain (red).~
Flow cytometry staining of PFA-fixed human Jukat cells with CD45RO antibody (clone 190-2F2.5); Red=

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NSJ-V2416SAF-100UG100 ug£534.00
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Further Information

Application Details:
Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml
Application Note:
Due to variation in protocol and secondary antibody used, the CD45RO antibody may need to be titered for optimal performance.
Description:
Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Lentillectin purified glycoproteins obtained from HuPBL and from a CLL (B cell) were used as immunogen for this CD45RO antibody.
Limitation:
This CD45RO antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein G affinity chromatography
Uniprot #:
P08575

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