NSJ Bioreagents

GFAP Antibody / Glial Fibrillary Acidic Protein (Antigen affinity purified)

Product Code:
 
NSJ-R30039
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the GFAP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE rat brain tissue with GFAP antibody (green), MBP antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
IHC staining of FFPE rat brain tissue with GFAP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 3
Western blot testing of 1) human U-251, 2) rat brain, 3) rat C6 and 4) mouse brain tissue with GFAP antibody. Predicted molecular weight ~50 kDa.
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Immunofluorescent staining of FFPE rat brain tissue with GFAP antibody (green), MBP antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE rat brain tissue with GFAP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human U-251, 2) rat brain, 3) rat C6 and 4) mouse brain tissue with GFAP antibody. Predicted molecular weight ~50 kDa.

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CodeSizePrice
NSJ-R30039-100ug100ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.4-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml (formalin or acetone fixed tissues),Immunofluorescence: 2-4ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the GFAP antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Glial fibrillary acidic protein is an intermediate filament protein of 52kDa. Its gene is mapped to human 17q21. GFAP is a useful marker of astroglia in the brain. Mutations in the protein are associated with Alexander disease.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Immunogen:
Glial fibrillary acidic protein from pig spinal cord was used as the immunogen for this GFAP antibody.
Limitation:
This GFAP antibody is available for research use only.
Purity:
Unpurified ascites

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