NSJ Bioreagents

CENPH Antibody / Centromere protein H (Antigen affinity purified)

Product Code:
 
NSJ-RQ6605
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the CENPH antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of human SiHa cells with CENPH antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CENPH antibody.~
2 / 2
Western blot testing of human 293T cells with CENPH antibody. Predicted mo
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Flow cytometry testing of human SiHa cells with CENPH antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CENPH antibody.~
Western blot testing of human 293T cells with CENPH antibody. Predicted mo

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NSJ-RQ6605-100ug100ug£535.00
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Further Information

Application Details:
Western blot: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the CENPH antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Centromere protein H is a protein that in humans is encoded by the CENPH gene. Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids E37-M247) was used as the immunogen for the CENPH antibody.
Limitation:
This CENPH antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q9H3R5

Documents