NSJ Bioreagents

Pyruvate Kinase Antibody / PKM2 (Antigen affinity purified)

Product Code:
 
NSJ-RQ6751
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Pyruvate Kinase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 5
Immunofluorescent staining of FFPE human A549 cells with Pyruvate Kinase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 5
IHC staining of FFPE differentiated adenocarcinoma of the human rectum tissue with Pyruvate Kinase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human liver cancer tissue with Pyruvate Kinase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of human 1) HeLa, 2) Jurkat and 3) SH-SY5Y cell lysate with Pyruvate Kinase antibody. Predicted molecular weight ~58 kDa.
5 / 5
Flow cytometry testing of human HeLa cells with Pyruvate Kinase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Pyruvate Kinase antibody.
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Immunofluorescent staining of FFPE human A549 cells with Pyruvate Kinase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE differentiated adenocarcinoma of the human rectum tissue with Pyruvate Kinase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with Pyruvate Kinase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa, 2) Jurkat and 3) SH-SY5Y cell lysate with Pyruvate Kinase antibody. Predicted molecular weight ~58 kDa.
Flow cytometry testing of human HeLa cells with Pyruvate Kinase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Pyruvate Kinase antibody.

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NSJ-RQ6751-100ug100ug£535.00
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Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Pyruvate Kinase antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
PKM (Pyruvate Kinase, Muscle), also known as OIP-3, PK3 and PKM2, is an enzyme that in humans is encoded by the PKM gene. The activity of pyruvate kinase subtype M2 is increased by fructose 1, 6-bisphosphate (Fru-1, 6-P2). By in situ hybridization, Popescu and Cheng (1990) mapped the THBP1 gene to 15q24-q25. Ashizawa et al. (1991) manipulated the intracellular Fru-1, 6-P2 concentration in several mammalian cell lines, including human, by varying the glucose concentration in the media. Using a novel proteomic screen for phosphotyrosine-binding proteins, Christofk et al. (2008) observed that PKM2 binds directly and selectively to tyrosine-phosphorylated peptides.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
N-terminal region amino acids KDPVQEAWAEDVDLRVNFAMNVGKAR from the human protein were used as the immunogen for the Pyruvate Kinase antibody.
Limitation:
This Pyruvate Kinase antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Uniprot #:
P14618

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