NSJ Bioreagents

H3K9me3 Antibody / Trimethylated Histone H3 at Lysine 9

Product Code:
 
NSJ-R20406
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
RM389
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Store the recombinant H3K9me3 antibody at -20°C.
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Western blot testing of recombinant histone H3.3 (lane 1) and acid extracts of HeLa cells (lane 2) with recombinant H3K9me3 at 0.5ug/ml showed a band of histone H3 trimethylated at Lysine 9 (K9me3) in HeLa cells.~
2 / 2
ELISA of designer nucle

Western blot testing of recombinant histone H3.3 (lane 1) and acid extracts of HeLa cells (lane 2) with recombinant H3K9me3 at 0.5ug/ml showed a band of histone H3 trimethylated at Lysine 9 (K9me3) in HeLa cells.~
ELISA of designer nucle

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NSJ-R20406-100UG100 ug£581.00
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details:
Western blot: 0.5ug/ml-2ug/ml,ELISA: 1ug/ml-10ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant H3K9me3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This antibody reacts to Histone H3 trimethylated at Lysine 9 (K9me3). No cross reactivity with monomethylated Lysine 9 (K9me1), dimethylated Lysine 9 (K9me2), or other methylations in histone H3.
Format:
Purified
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A trimethyl-peptide corresponding to Trimethyl-Histone H3 (Lys9) was used as the immunogen for the recombinant H3K9me3 antibody.
Limitation:
This recombinant H3K9me3 antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P84243

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