AdipoGen Life Sciences

Asprosin (human) (rec.) (His)

Product Code:
 
AG-40B-0174
Product Group:
 
Other Proteins
Host Type:
 
E.coli
Regulatory Status:
 
RUO
Shipping:
 
BI
Storage:
 
Short term: +4°C. Long term: -20°C
 

No additional charges, what you see is what you pay! *

CodeSizePrice
AG-40B-0174-C01010 ug£175.00
Quantity:
AG-40B-0174-30103 x 10 ug£340.00
Quantity:
AG-40B-0174-C100100 ug£705.00
Quantity:
Prices exclude any Taxes / VAT
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: Switzerland.
Typical lead time: 7-10 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Alternate Names/Synonyms:
Fibrillin-1 C-terminal Cleavage Product; FBN1 C Terminal Cleavage Product
Concentration:
After reconstitution: for 10ug size: 0.1mg/ml, for 100ug size: 1 mg/ml
Endotoxin:
<0.01EU/ug purified protein (LAL test).
Formulation:
Lyophilized from 0.2um-filtered solution in 50mM Tris-HCl (pH8.2), 200mM NaCl.
Handling Advice:
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution.
Long Description:
Asprosin is a new fasting-induced protein hormone that targets the liver to increase plasma glucose levels. Asprosin is the C-terminal cleavage product of the protein pro-Fibrillin-1. Asprosin is secreted from white adipose tissue and increases hepatic glucose production by using cAMP as a second messenger, leading to activation of protein Kinase A. Reduction of Asprosin levels protects against metabolic syndrome-associated hyperinsulinism. Asprosin may act as a circulating hunger signal. Indeed, peripherally injected recombinant asprosin can cross the blood-brain barrier and intracerebroventricular (i.c.v.) injection of recombinant asprosin stimulated appetite in wild-type mice, indicating a central mechanism of action. Loss of Asprosin in mice and human leads to decreased fat mass and body weight, and hypophagia. Mice are also completely protected from the development of diet-induced obesity. Asprosin works by stimulating the orexigenic AgRP+ (Agouti related neuropeptide) neurons via a cAMP-dependent pathway and by inhibiting the anorexigenic neurons POMC+ (pro-opiomelanocortin) neurons in a GABA-dependent manner. Mutation in Asprosin in human leads to the pattern of metabolic dysregulation, including partial lipodystrophy, accompanied by reduced plasma insulin. Due to its key role in food regulation, Asprosin function could serve as a potentially unique therapeutic target against obesity, diabetes or metabolic diseases. Endogenous asprosin runs on SDS-PAGE at ~30kDa, while bacterially expressed recombinant asprosin runs at 18kDa. The difference of migration is probably due to predicted three N-linked glycosylation sites and potentially other post-translational modifications that are lacking in bacteria. Bacterially expressed recombinant asprosin retains the biological activity displayed by its endogenously expressed counterpart.
Molecular Weight:
~18kDa (SDS-PAGE)
NCBI Uniprot Number:
P35555
Other Data:
Reconstitute with 100ul sterile water.
Product Description:
The protein Fc (LALA-PG)-KIH (human) IgG1 Control (rec.)?is produced by using two different vectors, one encoding for the Fc (LALA-PG) Knobs sequence with a small linker (synthesizing a protein of 29kDa) and one encoding for the Fc (LALA-PG) Holes sequence (synthesizing a protein of 28kDa). Both vectors transfected into HEK293 cells produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng.?9,?617 (1996)) required for dimerization and for secretion of the final protein?Fc (LALA-PG)-KIH (human) IgG1 Control (rec.).?The LALA-PG mutations inhibit binding to FcγRs and?C1q?while FcRn binding and Fc stability remain unaffected.
Purity:
>95% (SDS-PAGE)
Sequence:
Human asprosin (aa 2732-2871) is fused at the C-terminus to a His-tag.
Source / Host:
E. coli
Species Crossreactivity:
Human
Use & Stability:
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.