BAFF (human):Fc (human) (rec.)

BLyS; TALL-1; CD257; B Cell Activating Factor; TNFSF13B; Tumor Necrosis Factor Ligand Superfamily Member 13B

Rescues the production of mature follicular and marginal zone B cells. - in vitro: Addition of 1-100 ng/ml of Fc-BAFF induces B cell survival in a dose-dependent manner. - in vivo: Fc-BAFF treatment rescues mature B cell development in BAFF deficient mice. Mice are injected at day 0 with 100 µg Fc-BAFF i.v. and at day 14 with 50 µg Fc-BAFF i.p.

After reconstitution:for 10µg size: 0.1mg/mlfor 500µg size: 1 mg/ml

32160000

<0.01EU/µg purified protein (LAL test; Lonza).

liquid

Lyophilized. Contains PBS.

After reconstitution, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.Centrifuge lyophilized vial before opening and reconstitution.PBS containing at least 0.1% BSA should be used for further dilutions.

Protein. The receptor-binding domain of human BAFF (aa 136-285) is fused at the N-terminus to the Fc portion of human IgG1. Source: CHO cells. Endotoxin content: <0.01EU/µg purified protein (LAL test; Lonza). Lyophilized. Contains PBS. Binds to human and mouse BAFF-R, TACI and BCMA. Purity: >95% (SDS-PAGE). BAFF is mainly produced by innate immune cells such as neutrophils, monocytes, macrophages, dendritic cells, follicular dendritic cells. T cells, activated B cells, some malignant B cells and also non-lymphoid cells like astrocytes, synoviocytes and epithelial cells can also produce BAFF. BAFF binds three distinct receptors (BAFF-R, TACI and BCMA) expressed predominantly on B cells, although activated T cells also express BAFF-R. BAFF is a master regulator of peripheral B cell survival, and together with IL-6, promotes Ig class-switching and plasma cell differentiation. Besides its major role in B cell biology, BAFF co-stimulates activated T cells. Deregulated expression of BAFF leads to autoimmune disorders in mice. In humans, elevated levels of soluble BAFF have been detected in the serum of patients with various autoimmune diseases such as Sjoegren syndrome, Rheumatoid arthritis (RA), Multiple sclerosis (MS) and Systemic Lupus Erythematosus (SLE). BAFF has also increased levels in some lymphoid cancers.

~50kDa (SDS-PAGE; reducing conditions)~100kDa (SDS-PAGE; non-reducing conditions)~300kDa (SDS-PAGE; native conditions; multimerizes and forms hexamers)

Q9Y275

Plastic Vial

BAFF is mainly produced by innate immune cells such as neutrophils, monocytes, macrophages, dendritic cells, follicular dendritic cells. T cells, activated B cells, some malignant B cells and also non-lymphoid cells like astrocytes, synoviocytes and epithelial cells can also produce BAFF. BAFF binds three distinct receptors (BAFF-R, TACI and BCMA) expressed predominantly on B cells, although activated T cells also express BAFF-R. BAFF is a master regulator of peripheral B cell survival, and together with IL-6, promotes Ig class-switching and plasma cell differentiation. Besides its major role in B cell biology, BAFF co-stimulates activated T cells. Deregulated expression of BAFF leads to autoimmune disorders in mice. In humans, elevated levels of soluble BAFF have been detected in the serum of patients with various autoimmune diseases such as Sjoegren syndrome, Rheumatoid arthritis (RA), Multiple sclerosis (MS) and Systemic Lupus Erythematosus (SLE). BAFF has also increased levels in some lymphoid cancers.

>95% (SDS-PAGE)

The receptor-binding domain of human BAFF (aa 136-285) is fused at the N-terminus to the Fc portion of human IgG1.

CHO cells

Binds to human and mouse BAFF-R, TACI and BCMA.

Fc

Non-hazardous

Other Proteins

12352202

Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.