AdipoGen Life Sciences

Flagellin (rec.)

Product Code:
 
AG-40B-0125
Product Group:
 
Recombinant Proteins
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Shipping:
 
Blue Ice
Storage:
 
+4°C
1 / 1
Flagellin (rec.) (AG-40B-0125) and Flagellin (NLRC4 Mutant) (rec.) (AG-40B-0126) induce CCL20-luciferase promoter through TLR5 in vitro. Method: Caco-2 cells transfected with plasmid encoding CCL20-LUC plasmid are activated for 24h wit
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Flagellin (rec.) (AG-40B-0125) and Flagellin (NLRC4 Mutant) (rec.) (AG-40B-0126) induce CCL20-luciferase promoter through TLR5 in vitro. Method: Caco-2 cells transfected with plasmid encoding CCL20-LUC plasmid are activated for 24h wit

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AG-40B-0125-C01010 ug£160.00
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AG-40B-0125-30103 x 10 ug£330.00
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Further Information

Alternate Names/Synonyms:
FliC
Biological Activity:
Activation of TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, in vivo on expression of CCL20 or IL-6. TLR5 can be stimulated in vitro by Flagellin (rec.) at concentration range of 5-50ng/ml and in vivo at a starting concentration of 0.1-1µg/mouse. Inflammasome NLRC4 can be activated at a concentration range of 1-5µg/ml on bone-marrow macrophages in the presence of streptolysin O (SLO, 25µg/ml), a protein that allows delivery of exogenous molecules into the cytosol of living cells.
Concentration:
0.1mg/ml after reconstitution.
EClass:
32160000
Endotoxin:
<0.01EU/µg purified protein (LAL test).
Form (Short):
liquid
Formulation:
Lyophilized. Contains PBS.
Handling Advice:
After reconstitution, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.Centrifuge lyophilized vial before opening and reconstitution.
Long Description:
Protein. The native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495) is fused at the N-terminus to a His-tag. Source: E. coli. Endotoxin content: <0.01EU/µg purified protein (LAL test; Lonza). Lyophilized. Contains PBS. Detected by human and mouse TLR5 (Toll-like receptor 5) and mouse NLRC4 (Nod-like receptor [NLR] family caspase activation and recruitment domain [CARD] domain-containing protein 4). Purity: >95% (SDS-PAGE). Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) and the intracellular protein NLRC4 (NLR family CARD domain-containing protein 4).
Molecular Weight:
~54kDa (SDS-PAGE)
NCBI, Uniprot Number:
P06179
Package Type:
Plastic Vial
Product Description:
Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) and the intracellular protein NLRC4 (NLR family CARD domain-containing protein 4).
Purity:
>95% (SDS-PAGE)
Sequence:
The native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495) is fused at the N-terminus to a His-tag.
Source / Host:
E. coli
Specificity:
Detected by human and mouse TLR5 (Toll-like receptor 5) and mouse NLRC4 (Nod-like receptor [NLR] family caspase activation and recruitment domain [CARD] domain-containing protein 4).
TAGs:
His
Transportation:
Non-hazardous
UNSPSC Category:
Other Proteins
UNSPSC Number:
12352202
Use & Stability:
Stable for at least 6 months after receipt when stored at -20°C.