ProSci

MyD88 Monoclonal Antibody

Product Code:
 
PSI-PM-4673
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
2E9C2
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
1 / 2
Western blot analysis of MyD88 in EL4 whole cell lysate with MyD88 antibody at 2 μg/mL.
2 / 2
Immunohistochemistry of MyD88 in human heart with MyD88 antibody at 2.5 μg/mL.
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Western blot analysis of MyD88 in EL4 whole cell lysate with MyD88 antibody at 2 μg/mL.
Immunohistochemistry of MyD88 in human heart with MyD88 antibody at 2.5 μg/mL.

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CodeSizePrice
PSI-PM-4673-0.02mg0.02mg£150.00
Quantity:
PSI-PM-4673-0.1mg0.1mg£515.00
Quantity:
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Further Information

Additional Names:
MyD88 Antibody [2E9C2] : MYD88D
Application Note:
MyD88 antibody can be used for detection of MyD88 by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Background:
MyD88 Monoclonal Antibody: The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. MyD88 has been identified as an adapter molecule in the IL-1 signaling pathway. MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and dominant negative form of MyD88 attenuates IL-1R-mediated NF-κB activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor, which are members in the Toll/IL-1R family of receptors. Targeted disruption of the MyD88 gene results in lose of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues.
Background References:
  • Muzio M, Ni J, Feng P, et al. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling. Science 1997; 278:1612-5.
  • Adachi O, Kawai T, Takeda K, et al. Targeted disruption of the MyD88 gene results in loss of IL-1- and IL-18-mediated function. Immunity 1998; 9:143-50.
  • Medzhitov R, Preston-Hurlburt P, Kopp E, et al. MyD88 is an adaptor protein in the hToll/IL-1 receptor family signaling pathways. Mol. Cell 1998; 2:253-8.
  • Kawai T, Adachi O, Ogawa T, et al. Unresponsiveness of MyD88-deficient mice to endotoxin. Immunity 1999; 11:115-22.
Buffer:
MyD88 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Mouse monoclonal MyD88 antibody was raised against a recombinant protein corresponding to amino acids 176 to 280 of human MyD88.
NCBI Gene ID #:
4615
NCBI Official Name:
myeloid differentiation primary response gene (88)
NCBI Official Symbol:
MYD88
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
AAC50954
Protein GI Number:
1814020
Purification:
MyD88 Monoclonal Antibody is Protein A purified.
Research Area:
Innate Immunity
Swissprot #:
Q99836
User NOte:
Optimal dilutions for each application to be determined by the researcher.