Further Information
CD4 Antibody [9H5A8] : CD4mut
WB: 0.5-4 μg/mL; IHC: 5 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples. All other applications and species not yet tested.
CD4 Monoclonal Antibody: CD4 is a member of the immunoglobulin superfamily and is implicated as associative recognition element in MHC (Major Histocompatibility Complex) class II-restricted immune response. On T-lymphocytes, it defines the helper/inducer subset. The mature 55 kd CD4 protein consists of a 372 amino acid extracellular segment composed of four tandem immunoglobulin-like VJ regions. The CD4 molecule is a major receptor for human immunodeficiency virus (HIV), binding directly to the envelope glycoprotein gp120 on HIV, with the co-receptors being CCR5 or CXCR4. It has been shown that the V-like domains are critical for binding with HIV envelope gp120.
- Bowers et al. Int. J. Biochem. Cell Biol. 1997; 29 :871-5.
- Arthos et al. Cell 1989; 57:469-81.
CD4 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Mouse monoclonal CD4 antibody was raised against a 193 amino acid recombinant protein from near the amino terminus of human CD4.
Human CD4 has one isoform (458aa, 51kD). Mouse CD4 has two isoforms, including isoform 1 (457aa, 51kD) and isoform 2 (217aa, 24.5kD). Rat CD4 has one isoform (457aa, 51kD). PM-5201 can detect human, mouse and rat.
920
CD4 molecule
CD4
homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 51kD
Observed: 51-57 kD (Post-modyfication: 2-4 N-linked glycosylations)
NP_000607
10835167
CD4 Monoclonal Antibody is immunoaffinity chromotography purified IgG.
Immunology
P01730
Optimal dilutions for each application to be determined by the researcher.
Recombinant Protein Test (Figure 1): Anti-CD4 antibodies (PM-5201) detected human CD-4 recombinant protein at 5ng.