Further Information
APP Antibody: AAA, AD1, PN2, ABPP, APPI, CVAP, ABETA, PN-II, CTFgamma, A4, Amyloid beta A4 protein, APP
APP antibody can be used for detection of APP and the C99 fragment by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
APP Antibody: Accumulation of the amyloid-beta peptide (Abeta) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. The beta-amyloid protein precursor (APP) is cleaved by beta-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide (Abeta), which is deposited in the brains of all suffers of Alzheimer's disease.
- Ponte P, Gonzalez-DeWhitt P, Schilling J, Miller J, Hsu D, Greenberg B, Davis K, Wallace W, Lieberburg I, Fuller F. A new A4 amyloid mRNA contains a domain homologous to serine proteinase inhibitors. Nature 1988;331:525-7
- Selkoe D.J. Cell biology of the amyloid β-protein precursor and the mechanism of Alzheimer's disease.Annu Rev Cell Biol 1994;10:373-403 (RD1299)
APP Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/ml
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Pig: (100%), Guinea pig: (100%)
APP antibody was raised against a 15 amino acid peptide near the carboxy terminus of human APP.
The immunogen is located within the last 50 amino acids of APP.
351
amyloid beta (A4) precursor protein
APP
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 83 kDa
Observed: 110 kDa?
CAA30050
28721
APP Antibody is affinity chromatography purified via peptide column.
Neuroscience
P05067
Optimal dilutions for each application to be determined by the researcher.