ProSci

GOLPH2 Antibody

Product Code:
 
PSI-5441
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
1 / 3
Western blot analysis of GOLPH2 in rat brain tissue lysate with GOLPH2 antibody at (A) 0.25 and (B) 0.5 μg/mL.
2 / 3
Immunohistochemistry of GOLPH2 in rat brain tissue with GOLPH2 antibody at 2.5 μg/mL.
3 / 3
Immunofluorescence of GOLPH2 in rat brain tissue with GOLPH2 antibody at 20 μg/mL.
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Western blot analysis of GOLPH2 in rat brain tissue lysate with GOLPH2 antibody at (A) 0.25 and (B) 0.5 μg/mL.
Immunohistochemistry of GOLPH2 in rat brain tissue with GOLPH2 antibody at 2.5 μg/mL.
Immunofluorescence of GOLPH2 in rat brain tissue with GOLPH2 antibody at 20 μg/mL.

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CodeSizePrice
PSI-5441-0.02mg0.02mg£150.00
Quantity:
PSI-5441-0.1mg0.1mg£449.00
Quantity:
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Further Information

Additional Names:
GOLPH2 Antibody: GP73, HEL46, GOLPH2, C9orf155, PSEC0257, bA379P1.3, PSEC0242, UNQ686/PRO1326, Golgi membrane protein 1, Golgi membrane protein GP73
Application Note:
GOLPH2 antibody can be used for detection of GOLPH2 by Western blot at 0.25 - 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
GOLPH2 Antibody: GOLPH2, also known as GOLM1, is a Golgi phosphoprotein that has a short cytoplasmic N-terminal domain, a membrane spanning region, and a longer C-terminal domain. It was initially identified as a possible marker for Alzheimer's disease, although later studies have demonstrated that the GOLPH2 gene does not contribute to risk of this disease. GOLPH2 expression has been reported higher in prostate cancer tissues compared to normal prostate tissue, suggesting that GOLPH2 can be used as an additional positive marker for tissue-based diagnosis of prostate cancer. It has been suggested that GOLPH2 expression in hepatocellular carcinomas (HCCs) and serum may also serve as tumor markers for HCCs.
Background References:
  • Kladney RD, Bulla GA, Guo L, et al. GP73, a novel Golgi-localized protein upregulated by viral infection. Gene 2000; 249:53-65.
  • Li H, Wetten S, Li L, et al. Candidate single-nucleotide polymorphisms from a genomewide association study of Alzheimer disease. Arch. Neurol. 2008; 65:45-53.
  • Antunez C, Boada M, Lopez-Arrieta, et al. GOLPH2 gene markers are not associated with Alzheimer's disease in a sample of the Spanish population. J. Alzheimers Dis. 2009; 18:751-4.
  • Kristiansen G, Fritzsche FR, Wassermann K, et al. GOLPH2 protein expression as a novel tissue biomarker for prostate cancer: implications for tissue-based diagnostics. Br. J. Cancer 2008; 99:939-48.
Buffer:
GOLPH2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
GOLPH2 antibody was raised against an 18 amino acid synthetic peptide from near the center of human GOLPH2.

The immunogen is located within amino acids 130 - 180 of GOLPH2.
NCBI Gene ID #:
51280
NCBI Official Name:
golgi membrane protein 1
NCBI Official Symbol:
GOLM1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 67 kDa

Observed: 70 kDa
Protein Accession #:
EAW62705
Protein GI Number:
119583109
Purification:
GOLPH2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer
Swissprot #:
Q8NBJ4
User NOte:
Optimal dilutions for each application to be determined by the researcher.