ProSci

NOD3 Antibody

Product Code:
 
PSI-5949
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
1 / 2
Western blot analysis of NOD3 in 3T3 cell lysate with NOD3 antibody at (A) 1 and (B) 2 μg/mL.
2 / 2
Immunofluorescence of NOD3 in Jurkat cells with NOD3 antibody at 20 μg/mL.
❮❯

Western blot analysis of NOD3 in 3T3 cell lysate with NOD3 antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of NOD3 in Jurkat cells with NOD3 antibody at 20 μg/mL.

No additional charges, what you see is what you pay! *

CodeSizePrice
PSI-5949-0.02mg0.02mg£150.00
Quantity:
PSI-5949-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Related Products
  • Show All

Further Information

Additional Names:
NOD3 Antibody: NOD3, CLR16.2, NOD3, Protein NLRC3, CARD15-like protein
Application Note:
NOD3 antibody can be used for detection of NOD3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
NOD3 Antibody: NOD3 is a member of the NOD (nucleotide-binding oligomerization domain) family, a group of proteins that are involved in innate immune defense. NOD3 also known as NLR family CARD containing 3 (NLRC3), is predominantly expressed in the immune system, particularly in T lymphocytes, and its expression is strongly down-regulated following stimulation of the T-cell receptor complex and CD28, suggesting that NOD3 plays a role in attenuating the activation of T cells. NOD3 inhibits NF-kappaB, AP-1 and NFAT transcriptional activation in Jurkat T cells downstream of CD3/CD28 stimulation or treatment with PMA/ionomycin and decreases IL-2 and CD25 mRNA induction in activated cells.
Background References:
  • Kufer TA, Banks DJ, and Philpott DJ. Innate immune sensing of microbes by Nod proteins. Ann. NY Acad. Sci.2006; 1072:19-27.
  • Conti BJ, Davis BK, Zhang J, et al. CATERPILLER 16.2 (CLR16.2), a novel NBD/LRR family member that negatively regulates T cell function. J. Biol. Chem.2005; 280:18375-85.
Buffer:
NOD3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
NOD3 antibody was raised against an 18 amino acid synthetic peptide near the center of human NOD3.

The immunogen is located within amino acids 630 - 680 of NOD3.
NCBI Gene ID #:
197358
NCBI Official Name:
NLR family, CARD domain containing 3
NCBI Official Symbol:
NLRC3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
EAW85351
Protein GI Number:
119605757
Purification:
NOD3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Innate Immunity
Swissprot #:
Q7RTR2
User NOte:
Optimal dilutions for each application to be determined by the researcher.