Further Information
NALP12 Antibody: RNO, PAN6, RNO2, FCAS2, NALP12, PYPAF7, CLR19.3, RNO, NACHT, LRR and PYD domains-containing protein 12, Monarch-1
NALP12 antibody can be used for detection of NALP12 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
NALP12 Antibody: NALP proteins are cytoplasmic proteins that form a subfamily within the larger CATERPILLER family and are thought to play a crucial role in cell proliferation and reproduction. Like all other NALP family members, NALP12, also known as Monarch-1, has a C-terminal leucine-rich repeat (LRR) region, an N-terminal Pyrin domain (PYD) followed by a NACHT domain, and a NACHT-associated domain. NALP12 is thought to act as an attenuating factor of inflammation by suppressing inflammatory responses such as NF-κB activation by TLR-signaling molecules MyD88, IRAK-1, TRAF6 and RIPK1 in activated monocytes. Recent evidence suggests that mutations in NALP12 result in hereditary periodic fever syndromes.
- Tschopp J, Martinon F, and Burns K. NALPs: a novel protein family involved in inflammation. Nat. Rev. Mol. Cell Biol.2003; 4:95-104.
- Tian X, Pascal G, and Monget P. Evolution and functional divergence of NLRP genes in mammalian reproductive system. BMC Evol. Biol.2009; 9:202.
- Williams KL, Lich JD, Duncan JA, et al. The CATERPILLER protein Monarch-1 is an antagonist of toll-like receptor- , tumor necrosis factor a-, and Mycobacterium tuberculosis-induced pro-inflammatory signals. J. Biol. Chem.2005; 48:39914-24.
- Jeru I, Duquesnoy P, Fernandes-Alnemri T, et al. Mutations in NALP12 cause hereditary periodic fever syndromes. Proc. Natl. Acad. Sci. USA2008; 105:1614-9.
NALP12 Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
NALP12 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human NALP12.
The immunogen is located within amino acids 110 - 160 of NALP12.
91662
NLR family, pyrin domain containing 12
NLRP12
Homo sapiens
Liquid
NP_653288
21955154
NALP12 Antibody is affinity chromatography purified via peptide column.
Innate Immunity
P59046
Optimal dilutions for each application to be determined by the researcher.