Chemodex

5-CFDA

Product Code:
 
CDX-C0039
Product Group:
 
Dyes, Stains, and Probes
Supplier:
 
Chemodex
Regulatory Status:
 
RUO
Shipping:
 
Ambient
Storage:
 
+4 °C
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Chemical Structure
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Chemical Structure

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CDX-C0039-M02525 mg£74.00
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CDX-C0039-M100100 mg£196.00
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CDX-C0039-G0011 g£1,246.00
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Further Information

Alternate Names/Synonyms:
5-Carboxyfluorescein diacetate
Appearance:
White to yellow solid.
CAS:
79955-27-4
EClass:
32160000
Form (Short):
liquid
Handling Advice:
Protect from light and moisture.
InChi:
InChI=1S/C25H16O9/c1-12(26)31-15-4-7-19-21(10-15)33-22-11-16(32-13(2)27)5-8-20(22)25(19)18-6-3-14(23(28)29)9-17(18)24(30)34-25/h3-11H,1-2H3,(H,28,29)
InChiKey:
WPUZGNPQMIWOHE-UHFFFAOYSA-N
Long Description:
Chemical. CAS: 79955-27-4. Formula: C25H16O9. MW: 460.39. Synthetic. Carboxyfluorescein diacetate (CFDA), which was originally used to measure intracellular pH but was soon adapted for use as a cell viability indicator. Upon hydrolysis by intracellular nonspecific esterases, CFDA forms carboxyfluorescein. As compared with fluorescein, carboxyfluorescein contains extra negative charges and is therefore better retained in cells CFDA is moderately permeant to most cell membranes, with uptake greater at pH 6.2 than at pH 7.41. CFDA has been used as a viability probe with a variety of cells, including bacteria, fungi (e.g. Saccharomyces cerevisiae), spermatozoa, natural killer (NK) cells and tumor cells.
MDL:
MFCD00036872
Molecular Formula:
C25H16O9
Molecular Weight:
460.39
Package Type:
Vial
Product Description:
Carboxyfluorescein diacetate (CFDA), which was originally used to measure intracellular pH but was soon adapted for use as a cell viability indicator. Upon hydrolysis by intracellular nonspecific esterases, CFDA forms carboxyfluorescein. As compared with fluorescein, carboxyfluorescein contains extra negative charges and is therefore better retained in cells CFDA is moderately permeant to most cell membranes, with uptake greater at pH 6.2 than at pH 7.41. CFDA has been used as a viability probe with a variety of cells, including bacteria, fungi (e.g. Saccharomyces cerevisiae), spermatozoa, natural killer (NK) cells and tumor cells.
Purity:
>95% (HPLC)
SMILES:
CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC(=C3)C(O)=O)C1=CC=C(OC(C)=O)C=C1O2
Solubility Chemicals:
Soluble in DMSO.
Source / Host:
Synthetic.
Transportation:
Non-hazardous
UNSPSC Category:
Fluorescent Reagents
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at +4°C.

References

(1) J. Immun. Methods 172, 115 (1994) | (2) Anticancer Res. 14, 927 (1994) | (3) Appl. Environ Microbiol. 60, 1467 (1994) | (4) Cytometry 15, 213 (1994) | (5) Biol. Reprod. 34, 127 (1986) | (6) Immun. Lett. 2, 187 (1981)