ProSci

BCMA (Vicky-1) Monoclonal Antibody

Product Code:
 
PSI-XA-1021
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rat
Antibody Isotype:
 
Rat? IgG1
Antibody Clonality:
 
Monoclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Flow Cytometry
  • Immunocytochemistry (ICC)
Storage:
 
BCMA antibody can be stored at 4˚C, stable for one year.
 

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PSI-XA-1021-0.1mg0.1mg£729.00
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Further Information

Application Note:
BCMA antibody can be used to recognize human BCMA by Flow Cytometry and immunocytochemistry. Method: 3 days after transfection of cells with the indicated constructs, cells were fixed with acetone or 4% formaldehyde for 5min at RT. Slides were blocked with normal IgG, and incubated for 1 hour with 5 μg/mL Vicky-1 in 1%BSA / 1x PBS. After washes in PBS, samples were incubated with the secondary antibody for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC. Method: HEK 293 cells were mock transfected?or transfected with an expression plasmid coding for human BCMA. Cells (5x105) were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1 μg/mL of monoclonal antibody to BCMA (Vicky-1). After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and analyzed by flow cytometry. This product is for research use only.
Background References:
  • BAFF binds to the tumor necrosis factor receptor-like molecule B cell maturation antigen and is important for maintaining the peripheral B cell population: J.S. Thompson, et al.; J. Exp. Med. 192, 129 (2000).
  • BAFF AND APRIL: a tutorial on B cell survival: F. Mackay, et al.; Annu. Rev. Immunol. 21, 231 (2003).
Buffer:
PBS containing 0.02% sodium azide
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
BCMA (monoclonal Vicky-1) antibody was raised against recombinant human BCMA:Fc.
NCBI Gene ID #:
608
NCBI Official Name:
tumor necrosis factor receptor superfamily, member 17
NCBI Official Symbol:
TNFRSF17
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
BCMA (monoclonal Vicky-1) antibody was purified to 95% in SDS-PAGE.
Research Area:
Cytokine,Cancer
Swissprot #:
Q02223
User NOte:
Optimal dilutions for each application to be determined by the researcher.