Further Information
PKR, PRKR, EIF2AK1, C1orf24, NIBAN, GIG39, ADRB2, E2AK2, EIF2AK2, EIF2aK
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.
- Abujiang Pataer, et, al. (2002) Cancer Res; 62: 2239. K. D. Ryman, et, al. (2005) J. Virol; 79: 1487 - 1499. Susana Guerra, et, al. (2006) J. Biol. Chem; 281: 18734 - 18745.
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
PKR (Phospho-Thr446) antibody was raised against a peptide sequence around phosphorylation site of threonine 446 (K-R-T (p) -R-S) derived from Human PKR.
Phospho-Specific
5610
eukaryotic translation initiation factor 2-alpha kinase 2
EIF2AK2
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
68 kDa
NP_001129123.1
208431827
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Phospho-Specific
This antibody detects endogenous level of PKR only when phosphorylated at threonine 446.
P19525