ProSci

CDYL Antibody

Product Code:
 
PSI-45-035
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
45-035 staining (1ug/ml) of Human Testes lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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45-035 staining (1ug/ml) of Human Testes lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

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PSI-45-035-0.1mg0.1mg£626.00
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Further Information

Additional Names:
CDYL, chromodomain protein, Y-like, CDYL1, DKFZP586C1622, MGC131936, CDY-like, autosomal, bA620A17.2 (chromodomain protein, Y chromosome-like), chromodomain protein, Y chromosome-like, chromodomain protein, Y chromosome-like, isoform b, testis-specific chromodomain Y-like protein, DKFZp586C1622
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 75kDa band observed in Human Testes lysates (calculated MW of 66.5kDa according to NP_004815.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lahn et al , Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):8707-12; PMID: 12072557). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Lahn BT, Page DC. Retroposition of autosomal mRNA yielded testis-specific gene family on human Y chromosome. Nat Genet. 1999 Apr;21(4):429-33.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-SMLKYLQRKIDEF
NCBI Gene ID #:
9425
NCBI Official Name:
chromodomain protein, Y-like
NCBI Official Symbol:
CDYL
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004815.3, NP_001137442.1, NP_001137443.1
Protein GI Number:
25777617
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
This antibody is expected to recognize both eported isoforms NP_004815.3; NP_001137442.1; NP_001137443.1). Variants (NP_001137442.1; NP_001137443.1) encode the same isoform.
Swissprot #:
Q9Y232