ProSci

Brain Creatine Kinase Antibody

Product Code:
 
PSI-45-339
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
45-339 (0.1ug/ml) staining of human brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-339 (0.1ug/ml) staining of human brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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PSI-45-339-0.1mg0.1mg£626.00
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This product comes from: United States.
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Further Information

Additional Names:
CKB, brain creatine kinase, creatine kinase, brain , B-CK, CKBB , creatine kinase B-chain, creatine kinase-B
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 45-48kDa band observed in human brain lysates (calculated MW of 42.6kDa according to NP_001814.2). Recommended concentration: 0.1-0.3ug/ml.
Background References:
  • Balasubramani M, Day BW, Schoen RE, Getzenberg RH. Altered expression and localization of creatine kinase B, heterogeneous nuclear ribonucleoprotein F, and high mobility group box 1 protein in the nuclear matrix associated with colon cancer. Cancer Res. 2006 Jan 15;66(2):763-9.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-KPSDEHKTDLNPDN
NCBI Gene ID #:
1152
NCBI Official Name:
creatine kinase, brain
NCBI Official Symbol:
CKB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001814.2
Protein GI Number:
21536286
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
P12277