ProSci

Monoglyceride Lipase Antibody

Product Code:
 
PSI-45-896
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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45-896 (0.5ug/ml) staining of Human Frontal Cortex (A) and Mouse Adipose (B) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-896 (0.5ug/ml) staining of Human Frontal Cortex (A) and Mouse Adipose (B) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.

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PSI-45-896-0.1mg0.1mg£626.00
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Further Information

Additional Names:
monoglyceride lipase, HU-K5, MGLL, MGL, lysophospholipase-like
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 35kDa band observed in Human Brain (Frontal Cortex) and Mouse Adipose lysates (calculated MW of 33.3kDa according to NP_001003794.1). Recommended concentration: 0.5-1.5ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat for this particular batch.Immunohistochemistry:In paraffin embedded Human Liver, expression of MGLL is detected in hepatocytes. Recommended concentration: 5ug/ml.
Background References:
  • Blankman JL, Simon GM, Cravatt BF. A comprehensive profile of brain enzymes that hydrolyze the endocannabinoid 2-arachidonoylglycerol. Chem Biol. 2007 Dec;14(12):1347-56
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-QDLPHLVNADGQY
NCBI Gene ID #:
11343
NCBI Official Name:
monoglyceride lipase
NCBI Official Symbol:
MGLL
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_009214.1, NP_001003794.1
Protein GI Number:
6005786
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_009214.1; NP_001003794.1).
Swissprot #:
Q99685