ProSci

ZO-1 Antibody

Product Code:
 
PSI-42-230
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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42-230 (0.3ug/ml) staining of Human Cerebral Cortex lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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42-230 (0.3ug/ml) staining of Human Cerebral Cortex lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

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PSI-42-230-0.1mg0.1mg£626.00
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This product comes from: United States.
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Further Information

Additional Names:
TJP1, tight junction protein 1 (zona occludens 1), DKFZp686M05161, MGC133289, ZO-1, OTTHUMP00000174520, tight junction protein 1, tight junction protein ZO-1, zona occludens 1, zonula occludens 1 protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 170kDa band observed in Human Brain (Cerebral Cortex) lysates (calculated MW of 187kDa according to NP_783297.2). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Expression of the protein seen in the cell junctions of A431 cells. Recommended concentration: 10ug/ml.
Background References:
  • Zhong Z, Deane R, Ali Z, Parisi M, Shapovalov Y, O\1Banion MK, Stojanovic K, Sagare A, Boillee S, Cleveland DW, Zlokovic BV, ALS-causing SOD1 mutants generate vascular changes prior to motor neuron degeneration. Nat. Neurosci. 2008 Apr 11 (4): 420-2.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-PKPTSQNQFSEHDKT
NCBI Gene ID #:
7082
NCBI Official Name:
tight junction protein 1
NCBI Official Symbol:
TJP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003248.3, NP_783297.2
Protein GI Number:
116875767
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Membrane
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_003248.3; NP_783297.2)
Swissprot #:
Q07157