Further Information
SHC, SHCA, PTP2C, SHPTP2, SH2 domain protein C1, SHC, SHC-transforming protein 1, Src homology 2 domain-containing-transforming protein C1
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span
- Trampont P, et al. (2006) Mol Cell Biol; 26 (23): 9035-9044. Patrussi L, et al. (2005) Oncogene; 24 (13): 2218-2228van der Geer P, et al. (1996) Curr Biol ; 6 (11): 1435-1444
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shc1 (Phospho-Tyr427) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 427 (P-S-Y (p) -V-N derived from Human Shc1.
Phospho-Specific
6464
SHC (Src homology 2 domain containing) transforming protein 1
SHC1
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
46, 52 kDa
NP_001123512.1
194239664
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Phospho-Specific
This antibody detects endogenous level of Shc1 only when phosphorylated at tyrosine 427.
P29353