ProSci

TRAIL Monoclonal Antibody

Product Code:
 
PSI-XP-5726-M
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Clonality:
 
Monoclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Neutralisation
  • Western Blot (WB)
Storage:
 
Trail/Apo2L lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks a
1 / 1
This antibody stained formalin-fixed, paraffin-embedded sections of human renal cell carcinoma. The recommended concentration is 2.5 ug/ml- 1.5 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 9.0 buffer is recommended. Optimal concentrations and conditions may vary.
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This antibody stained formalin-fixed, paraffin-embedded sections of human renal cell carcinoma. The recommended concentration is 2.5 ug/ml- 1.5 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 9.0 buffer is recommended. Optimal concentrations and conditions may vary.

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CodeSizePrice
PSI-XP-5726-M-0.5mg0.5mg£488.00
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Further Information

Additional Names:
APO2L, Tumor Necrosis Factor (ligand) Superfamily Member 10, CD253, APO2L, TRAIL, Tumor necrosis factor ligand superfamily member 10, Apo-2 ligand, Apo-2L, TL2
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hTRAIL/Apo2L (10.0 ng/mL), a concentration of 0.30-0.60 μg/mL of this antibody is required.

ELISA:

In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human TRAIL/APO-II when used with our biotinylated antigen affinity purified anti-human TRAIL/APO-II (XP-5289Bt) as the detection antibody at a concentration of approximately 1.0-2.0 μg/mL.

Western Blot:

To detect hTRAIL/Apo2L by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTRAIL/Apo2L is 0.25-1.0 ng/lane, under reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
The monoclonal antibody was produced in Balb/c X ICR F1 mice using recombinant hTRAIL/APO-II as the immunizing antigen.
NCBI Gene ID #:
8743
NCBI Official Name:
tumor necrosis factor (ligand) superfamily, member 10
NCBI Official Symbol:
TNFSF10
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P50591
Protein GI Number:
1730015
Purification:
This IgG1k antibody was purified from ascites fluid on a Protein A affinity column.
Research Area:
Apoptosis,Cancer, Functional Assays
Swissprot #:
P50591
User NOte:
Centrifuge vial prior to opening.