ProSci

BID Antibody

Product Code:
 
PSI-45-021
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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45-021 (0.1ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-021 (0.1ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

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PSI-45-021-0.1mg0.1mg£626.00
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Further Information

Additional Names:
BID, BH3 interacting domain death agonist , HGNC:1050, MGC15319, MGC42355 , BH3-interacting domain death agonist, Human BID coding sequence, apoptic death agonist, FP497, BID isoform ES(1b), BID isoform L(2), BID isoform Si6, OTTHUMP00000196197, desmocollin type 4
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 26+24kDa bands observed in A431 cell lysates (calculated MW of 26.8kDa according to NP_932070.1 and 22.0kDa according to NP_001187.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence:?Strong expression of the protein seen in the cytoplasm of MCF7 cells. Recommended concentration: 10ug/ml.
Background References:
  • Lund T, Stokke T, Olsen OE, Fodstad O. Garlic arrests MDA-MB-435 cancer cells in mitosis, phosphorylates the proapoptotic BH3-only protein Bim(EL) and induces apoptosis. Br J Cancer. 2005 Apr 12;
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-TYVRSLARNGMD
NCBI Gene ID #:
637
NCBI Official Name:
BH3 interacting domain death agonist
NCBI Official Symbol:
BID
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_932070.1, NP_001187.1, NP_932071.1
Protein GI Number:
37574726
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Apoptosis ,Cancer
SPECIFICITY:
This antibody is expected to recognise all three reported isoforms (as represented by NP_932070.1; NP_001187.1; NP_932071.1)
Swissprot #:
P55957