Further Information
SERPINE1, PAI1, PAI, PAI-1, PLANH1, serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1, plasminogen activator inhibitor, type I, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1, plasminogen activator inhibitor-1
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 48kDa band observed in Human Liver and HeLa lysates, while approx. 45kDa observed in lyates of cell lines A549 and HepG2 (calculated MW of 45.1kDa according to NP_000593.1). The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Placenta shows strong staining of cytotrophoblasts. Recommended concentration: 4-6ug/ml.
A customer successfully used this product on Human Breast cancer.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant PAI1 captured by a rabbit antibody. Recommended reporter concentration: 1.5-3ug/ml
- Baumruker T, Csonga R, Pursch E, Pfeffer A, Urtz N, Sutton S, Bofill-Cardona E, Cooke M, Prieschl E. Activation of mast cells by incorporation of cholesterol into rafts. Int Immunol. 2003 Oct;15(10):1207-18.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Dog
The immunogen for this antibody is: C-GFKIDDKGMAPALRH
5054
serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
SERPINE1
Homo sapiens
Liquid
NP_000593.1
10835159
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Signal Transduction ,Obesity
.
P05121