ProSci

TECK Antibody

Product Code:
 
PSI-XP-5725-M
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Clonality:
 
Monoclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Neutralisation
  • Western Blot (WB)
Storage:
 
TECK lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4&#
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This antibody stained formalin-fixed, paraffin-embedded sections of human normal small bowel. The recommended concentration is 2.5 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen and a Proteinase K antigen retrieval. Optimal concentrations and conditions may vary.
2 / 2
This antibody stained formalin-fixed, paraffin-embedded sections of human normal small bowel. The recommended concentration is 2.5 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen and a Proteinase K antigen retrieval. Optimal concentrations and conditions may vary.
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This antibody stained formalin-fixed, paraffin-embedded sections of human normal small bowel. The recommended concentration is 2.5 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen and a Proteinase K antigen retrieval. Optimal concentrations and conditions may vary.
This antibody stained formalin-fixed, paraffin-embedded sections of human normal small bowel. The recommended concentration is 2.5 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen and a Proteinase K antigen retrieval. Optimal concentrations and conditions may vary.

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CodeSizePrice
PSI-XP-5725-M-0.5mg0.5mg£488.00
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Further Information

Additional Names:
Thymus expressed chemokine, Small inducible cytokine A25, CCL25, SCYA25, TECK, C-C motif chemokine 25, Chemokine TECKTECK, Ckb15
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hTECK (100 ng/mL), a concentration of 10.0-20.0 μg/mL of this antibody is required.

ELISA:

In a sandwich ELISA (assuming 100 μL/well), a concentration of 1.0-2.0 μg/mL of this antibody will detect at least 300 pg/mL of recombinant human TECK when used with our biotinylated antigen affinity purified anti-human TECK (XP-5280Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.

Western Blot:
To detect hTECK by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTECK is 0.50-1.0 ng/lane, under non-reducing conditions.
Background:
This gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. TECK displays chemotactic activity for dendritic cells, thymocytes, and activated macrophages but is inactive on peripheral blood lymphocytes and neutrophils. TECK binds to chemokine receptor CCR9. Two alternatively spliced variants encoding two different isoforms have been identified.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
The monoclonal antibody was produced in Balb/c X ICR F1 mice using recombinant hTECK as the immunizing antigen.
NCBI Gene ID #:
6370
NCBI Official Name:
chemokine (C-C motif) ligand 25
NCBI Official Symbol:
CCL25
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
O15444
Protein GI Number:
212286066
Purification:
This IgG1k antibody was purified from ascites fluid on a Protein A affinity column.
Research Area:
Immunology,Chemokines & Cytokines, Functional Assays
Swissprot #:
O15444
User NOte:
Centrifuge vial prior to opening.