ProSci

MIP-1a Antibody

Product Code:
 
PSI-XP-5734-M
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Clonality:
 
Monoclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Neutralisation
  • Western Blot (WB)
Storage:
 
MIP-1α lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks a
 

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PSI-XP-5734-M-0.5mg0.5mg£488.00
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Further Information

Additional Names:
Macrophage inflammatory protein 1 alpha, CCL 3, MIP 1 alpha, MIP1a, SCYA 3, Heparin binding chemotaxis protein, G0S19-1, MIP1A, SCYA3, C-C motif chemokine 3, Macrophage inflammatory protein 1-alpha, MIP-1-alpha,
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-1α (100 ng/mL), a concentration of 5.0-10.0 μg/mL of this antibody is required.

ELISA:

In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 2000 pg/mL of recombinant human MIP-1α when used with our biotinylated antigen affinity purified anti-human MIP-1α (XP-5232Bt) as the detection antibody at a concentration of approximately 1.0-2.0 μg/mL.

Western Blot:

To detect hMIP-1α by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-1α is 1.0-2.0 ng/lane, under non-reducing conditions.
Background:
Macrophage Inflammatory Protein 1 alpha and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
The monoclonal antibody was produced in C3H/HeJ mice using recombinant hMIP-1α as the immunizing antigen.
NCBI Gene ID #:
6348
NCBI Official Name:
chemokine (C-C motif) ligand 3
NCBI Official Symbol:
CCL3
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P10147
Protein GI Number:
127078
Purification:
This IgG1 antibody was purified from ascites fluid by SAS precipitation followed by ion exchange column.
Research Area:
Immunology,Chemokines & Cytokines, Functional Assays
Swissprot #:
P10147
User NOte:
Centrifuge vial prior to opening.